Atif O

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Skills & Interests

Skills and Interests are Increased from my Postgraduate Studies:

Molecular biology, Cancer studies, Human genetics, Genetic markers, Biology, Cell biology, Human stem cells, Human cancer stem cells, Genomics, Gene expression, Medical and molecular laboratories skills, Molecular cloning techniques, Polymerase chain reaction (PCR) technique, Reverse transcription polymerase chain reaction (RT-PCR) technique, Real-time quantitative reverse transcription PCR (Real-Time qRT-PCR) technique, DNA extraction and purification, DNA sequencing, RNA isolation and purification, RNA sequencing, Proteins extraction, Western blotting, Tissue culture, Gene knockdown technique, Analysis of genes and proteins in normal and cancerous cells, Meta-analysis software, Analysis of DNA and RNA quality, Stem cell markers analysis, Extreme limitations dilution (ELDA) analysis, Cell viability assay and differentiation of human cells.

Using Image Lab Software, Quantity One 1-D Analysis Software, Geneious SoftwareApE A plasmid Editor, SnapGene Software, NanoDrop Instrument, Quabit FluorometerCFX Manager Software, GNU Image Manipulation ProgramSnagit Software, ExPASy Translate web tool, THE HUMAN PROTEIN ATLAS WEB, NEBcutter web tool, Ensembl Genome Browser, Bioinformatics Tools for Pairwise Sequence Alignment, Global Sequence Alignment Tool, science gateway Walter+Eliza Hall Bioinformatics, CancerMA Analysis.   
 
Designing of PCR primers using the following web tools (i) Oligonucleotide Properties Calculator; (ii) Primer 3 Input; (iii) Primer 3 Plus.

I would like to offer special thanks to Dr. Ramsay McFarlane for all his guidance and advice. I would like to express my gratitude to Dr. David Pryce, Dr. Natalia Gomez Escobar and Dr. Jane Wakeman.  I also appreciate all my colleagues at Bangor University, as I am deeply grateful for all their assistance and support. 

Genomes and Molecular Genetics:

During my study of genomes and molecular genetics I learned much about the human genome structure and organisation, including the processes of DNA replication and transcription, and RNA translation. I became familiar with the principles and processes of molecular biology, such as epigenetic regulation, DNA damage and repair, the molecular mechanism of cell ageing and programmed cell death, as well as gene isolation and complementary DNA (cDNA). 

I am grateful to Dr. David Pryce for allowing me the opportunity to work with him, and for training me in the basics of medical molecular biology. I am grateful for his strong support.

Molecular Biology of Diseases:

Through the molecular biology of diseases module I gained an understanding of the structure and functions of cellular membranes, and of the cellular ageing processes. I now understand the biological basis of human blood types. I have a good understanding of the various roles of the Endoplasmatic Reticulum and the different structures and functions of the nucleus. 

In this module I studied the characteristics of cancer cells, learning about tumour suppressors and oncogenes, and coming to understand the molecular basis and symptoms of a range of diseases such as Werner Syndrome, Cystic Fibrosis, Zellweger Syndrome and Motor Neuron Disease. I learned how Lysosomes are linked with the Endoplasmatic Reticulum, and about Lysosomal Storage Disease. I also learned about the cellular defect which causes Hypercholesterolemia (Endocytosis Disorder). I studied Choroideremia (Vesicle Transport Disorder), trinucleotide repeat disorders, Huntington Disease, Fragile X Syndrome as well as Chromosomal Disorders. 

I want to thank Dr. Thomas Caspari for his great support and encouragement during this module.

Techniques of Molecular Biology and Biotechnology: 

The techniques of molecular biology and biotechnology module helped me to develop skills in writing practical reports, while teaching me how to extract a DNA and RNA. I learned how to analyse the DNA and RNA using agarose gel electrophoresis and other techniques, such as plasmid mapping. 

I want to thank Dr. Anil Shirsat for helping me to understand and apply this important information.

Molecular and Medical Laboratory Techniques:

The molecular and medical laboratory techniques module has given me the skills and techniques needed both in molecular biological research, and also in medical research. I enjoyed and fascinated by the cloning of genes and by the laboratory work we conducted. I not only learned the theory and utilisation of the polymerase chain reaction (PCR), but I learned how to organise my results into a structured and scientific report, and to analyse and interpret both quantitative and qualitative data. 

Again, I wish to thank Dr. David Pryce for his inspired teaching of this module.

Medical Biotechnology: 

The lectures and seminars on medical biotechnology provided me with a good understanding of the medical application of biotechnology. I found particularly interesting the topics regarding stem cells, tissue engineering, and molecular modelling for in silico drug discovery. I learned that Angiogenesis is a new frontier in cancer research, and about molecular advances in malaria research. 

I am grateful to Dr. Ramsay McFarlane for organising this module.

Human Immunology and Disease: 

In the human immunology and disease module I came to understand the role of the immune system in healing, and in the development of a range of disorders and diseases. I learned how immunology can be used to find cures and treatments for cancer and other diseases. I also learned about the role of biomarkers and the immune system in the diagnosis and management of disease. 

I am thankful to both Dr. David Pryce and Dr. Pearl Huey for their support and professionalism throughout this module.

Medical Microbes, Viruses and Parasites: 

In this module I learned about medical microbes, parasites, and viruses. I now understand the medical and molecular aspects of parasites, of toxins and infections, as well as of infectious diseases, using in vitro techniques. I also understand the need to identify the efficacy of antibiotics through measuring antibiotic susceptibility. 

My thanks go to Dr. Henk Braig who made his teaching on the morphology, and pathogenicity of bacteria so interesting and relevant.

Medical Applications of Genome Analysis: 

During the medical application of genome analysis module, I learnt many interesting things, such as gathering of data using the post-genomics technologies. I also learnt about the relevance of mathematics, in obtaining information from post-genomics technology. I learned about personalised medicine and about array technology in diagnostic medicine. I am now able to use bioinformatic tools to analyse data sets. 

I am very grateful to Dr. Mark Hooks for his teaching of this module, and for clarifying a potentially complex subject.

Bioscience Internet Tools: 

I learnt many interesting things, such as (i) How to use the National Center for Biotechnology Information (NCBI) web tool that is one of the most important web tools for any researcher who would like to research for scientific literature, proteins information or genes sequences. (ii) How to use the Polyview-3D web tool that is a great web tool for exploring protein structures "we only need the structure protein ID from the NCBI web page". (iii) How to use the Biology WorkBench web tool that is a collection of great molecular biology tool box. (iv) How to obtain proteins information quickly by using SMART web tool. (v) How to analysis protein-protein interactions in several organisms by using the Biological General Repository for Interaction Datasets (BioGRID) web tool.       

Again, I want to thank Dr. Thomas Caspari for providing me this module and for training me to understand these important Internet tools.

Natural Sciences: Research Skills: 

In the natural sciences research skills module I learned about Health & Safety policies and Environmental legislation, and about the obligations on both employers and employees. There are many biological and chemical hazards and that is why it is necessary to implement a strategy for risk control. In this model I also learned about statistics and about using the SPSS Statistics software, and I learned how to analyse and interpret data sets. 

I particularly want to thank Dr. John Latchford for his clear explanations.

E-learning Course on Getting Published in Science: 

This course encouraged me to publish; I learned much about (i) Copyright Issues, (ii) Journal Impact Factor, (iii) Endnote and Reference Manager, (iv) Author Lists. It provided me with free literature search engines and current research excellence framework guidelines.      

I am thankful to both Michele Underwood and Nicola Kirby for organising this course.

E-learning Course on Research Methods in Science: 

During this course, I learnt many things, for example, that scientific research includes extensive methods and approaches. This course gave me an outline of the field, from the early stages of framing the research question, through the research, to writing up the findings, and onwards to deciding the next steps.       

Again, I would like to thank both Michele Underwood and Nicola Kirby for organising this essential, fundamental course, necessary for any researcher.

General Skills:

Computer skills, Microsoft office, Humanitarian volunteer works, Excellent interpersonal and people management skills, Takes initiative, Able to work accurately or study under pressure, Proven ability to work on own initiative and as part of a team, Leadership skills including managing, developing and motivating teams to achieve targets, Patient and reliable, Cooperative and flexible in studying or working, Ability to accept and learn everything new in work or study.

Tabuk University in the Kingdom of Saudi Arabia
Bangor University in the United Kingdom
Umm-Al-Qura University in the Kingdom of Saudi Arabia

Email: info@atifoyouni.com
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